RAPID DIAGNOSTIC TEST FOR GENOTYPING

ETABLISSEMENT FRANCAIS DU SANG - 06/03/2021

Description

The French Blood Establishment laboratory has developed an innovative diagnostic test based on visual detection of multiplex PCR amplicons, for the identification of Single Nucleotide Polymorphisms (SNP) in biological samples. APPLICATIONS: • Diagnostic and therapeutic management: genotyping of SNPs associated with : Pathologies / Predisposition to pathologies / Pathologies severity /Therapeutic response of the patient / Blood group typing • Forensics: accurate identification of individuals • Agriculture and water quality

Benefits

• Rapid test : Processing time is 45 minutes to 1h30 • Simple protocol : PCR is directly performed on the biological sample • Direct analysis of the amplified products: no previous purification or denaturation steps is required • Multiplex : Several SNPs can be genotyped simultaneously

Innovations

This invention is based on a rapid test for the multiplex and visual detection of PCR products directly amplified from biological samples. This multiplex test allows simultaneous identification of several SNP alleles in a single reaction. This rapid test doesn’t require nucleic acid extraction steps of the biological sample, purification and denaturation of the amplified products. The proof of concept was achieved on human erythrocyte antigens of DUFFY, MNS and KIDD blood group systems by the simultaneous identification of blood group SNPs.

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