INNOVATIVE DIAGNOSTIC TOOLS FOR NUCLEIC ACID DETECTION BASED ON ULTRASENSITIVE POLYTHIOL PROBES

ETABLISSEMENT FRANCAIS DU SANG - 06/03/2021

Description

Despite considerable advances over the last years in diagnostic, innovative diagnostic system allowing a sensitive, specific, fast and multiplex detection of nucleic acids are still needed. In this context, the development of new diagnostic technologies with high performances appears to be a challenge. The EFS TransDiag Laboratory developed innovative diagnostic tool with very high performances dedicated to rapid analysis of genomes. APPLICATIONS: • Diagnostic : biological analysis and R&D • Quality control in: Environment - freshwater, sewage Security - freshwater, epidemic survey Industrial process - food, pharmaceutical, cosmetic industries

Benefits

• Robust molecular tool can be integrated in any kind of diagnostic platforms (optical and electrochemical) depending on targeted needs • Targets: detection of short/ long synthetic or natural amplified sequences • Ultrasensitive detection: 10 pM for optical detection and 10 fM for electrochemical detection • High specificity: DNA screening or sequencing • Multiplex: able to detect several nucleic acid sequences in parallel

Innovations

Development of robust polythiol probes which can be efficiently immobilized in a stable way on : • Maleimide activated microplates (ELOSA) • Electrodes (gold, diamond…..) • Magnetic/micro/nanoparticles These formats are compatible with both optical and electrochemical detection. These innovative molecular tools increased strongly the performances of analytical systems

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