Nanoparticle-Enhanced Dot Blot Diagnostic Method to Test for Campylobacter spp. In Poultry

INRAE Transfert - 04/12/2022

Description

Cost-effective, functionalized biotin silica nanoparticles (Si-NP) are coupled to a paper based DNA blot test to improve sensitivity of Campylobacter nucleic acid detection. Equally applicable to DNA extracted using existing methods and insensitive to DNA polymerase inhibitors, this test is functional in various food matrices. Not only does the probe enable a higher sensitivity of detection, the amount detected can also be quantified. This test drastically cuts time and increases precision which could better detect this bacterial strain or be adapted to other biological contaminants

Benefits

• Up to 300 times more sensitive than current dot-blot on the market thanks to a single additional step. • Detection levels of 3pg/μL of DNA (600 bacterial cells) • Drastic reduction in time when compared with ISO-certified methods (from 7 days to 1 day) • Competitive costs and easy to integrate into existing kits

Innovations

There are 3 possible solutions that could be developed depending upon the commercial strategy: • Reagent kit for end-users that already have lab equipment; • lab in a box with all necessary equipment within a compact, portable laboratory; • Lab-on-the-chip based biosensor for quick and easy, on-site testing with a smartphone application to decipher the results; this is a long-term solution and may require further collaboration efforts.

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