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- TRL (Technology readiness level) -
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- Titre Rapid diagnostic test for genotyping
- Description This invention concerns a radid test for the multiplex and visual detection genotyping of PCR products directly amplified from biological samples. This multiplex test allows simultaneous identification of several SNP alleles in a single reaction. This rapid test doesn’t require nucleic acid extraction steps of the biological sample, purification and denaturation of the amplified products.
The proof-of-concept was achieved on human erythrocyte antigens of DUFFY, MNS and KIDD blood group systems by the simultaneous identification of blood group SNPs.
- Bénéfices • Diagnostic and therapeutic management: genotyping of SNPs associated with :
Pathologies / Predisposition to pathologies / Pathologies severity / Therapeutic response of the patient / Blood group typing
• Forensics: accurate identification of individuals
- Nouveauté • Rapid test : Processing time is 45 minutes to 1h30.
• Simple protocol : PCR is directly performed on the biological sample.
• Direct analysis of the amplified products: no previous purification or
denaturation steps is required.
• Multiplex : Several SNPs can be genotyped simultaneously.
• Analysis of 25 to 500 nucleotides-long amplicons.
- Mots clés Genotyping, single-nucleotide polymorphism (SNP), nucleic acid probes, multiplex test, visual detection, rapid, low-cost
- Secteurs Health
