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- TRL (Technology readiness level) -
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- Titre INNOVATIVE DIAGNOSTIC TOOLS FOR NUCLEIC ACID DETECTION BASED ON ULTRASENSITIVE POLYTHIOL PROBES
- Description Despite considerable advances over the last years in diagnostic, innovative diagnostic system allowing a sensitive, specific, fast and multiplex detection of nucleic acids are still needed. In this context, the development of new diagnostic technologies with high performances appears to be a challenge. The EFS TransDiag Laboratory developed innovative diagnostic tool with very high performances dedicated to rapid analysis of genomes.
APPLICATIONS:
• Diagnostic : biological analysis and R&D
• Quality control in:
Environment - freshwater, sewage
Security - freshwater, epidemic survey
Industrial process - food, pharmaceutical, cosmetic industries
- Bénéfices • Robust molecular tool can be integrated in any kind of diagnostic platforms (optical
and electrochemical) depending on targeted needs
• Targets: detection of short/ long synthetic or natural amplified sequences
• Ultrasensitive detection: 10 pM for optical detection and 10 fM for electrochemical
detection
• High specificity: DNA screening or sequencing
• Multiplex: able to detect several nucleic acid sequences in parallel
- Nouveauté Development of robust polythiol probes which can be efficiently immobilized in a stable way on :
• Maleimide activated microplates (ELOSA)
• Electrodes (gold, diamond…..)
• Magnetic/micro/nanoparticles
These formats are compatible with both optical and electrochemical detection. These innovative molecular tools increased strongly the performances of analytical systems
- Mots clés Inn or screening, optical ovative diagnostic, polythiol probes, genotypingor electrochemical detection (Enzyme Linked OligoSorbent Assay ELOSA, sensors…).
- Secteurs Chemicals, Materials & plant-based materials Consumer Goods Health
